Recent Publications


H. Ryoo, R. Giovanni, H. Kimmel, I. Jain, GH Underhill. Combinatorial microgels for 3D ECM screening and heterogeneous microenvironmental culture of primary human hepatic stellate cells. Advanced Science. 2024.10.1002/advs.202303128

SG Zambuto, I. Jain, HS Theriault, GH Underhill, BAC Harley. Cell chirality of micropatterned endometrial microvascular endothelial cells. Advanced Healthcare Materials. 2024. 10.1002/adhm.202303928


H. Ryoo, H. Kimmel, E. Rondo, GH Underhill. Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications. Bioengineering & Translational Medicine.

I. Jain, A. Brougham-Cook, GH Underhill. Effect of distinct ECM microenvironments on the genome-wide chromatin accessibility and gene expression responses of hepatic stellate cells. Acta Biomaterialia. 2023.

H. Ryoo, GH Underhill. Spatially Defined Cell-Secreted Protein Detection Using Granular Hydrogels: μGeLISA. ACS Biomaterials Science & Engineering. 2023.


A Brougham-Cook, HRC Kimmel, CP Monckton, D Owen, SR Khetani, GH Underhill. Engineered matrix microenvironments reveal the heterogeneity of liver sinusoidal endothelial cell phenotypic responses. APL Bioengineering. 2022.

CP Monckton, A Brougham-Cook, GH Underhill, SR Khetani. Modulation of human iPSC-derived hepatocyte phenotype via extracellular matrix microarrays. Acta Biomaterialia. 2022.

I Jain, IC Berg, A Acharya, M Blaauw, N Gosstola, P Perez-Pinera, GH Underhill. Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation. Commun Biol. 2022.

SG Zambuto, I Jain, KBH Clancy, GH Underhill, BAC Harley. Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels. ACS Biomaterials Science & Engineering. 2022.

ST Berent, I Jain, GH Underhill, AM Wagoner Johnson. Simulated Confluence on Micropatterned Substrates Correlates Responses Regulating Cellular Differentiation. Biotechnology and Bioengineering. 2022.


A Brougham-Cook, I Jain, DA Kukla, F Masood, H Kimmel, H Ryoo, SR Khetani, GH Underhill. High Throughput Interrogation of Human Liver Stellate Cells Reveal Microenvironmental Regulation of Phenotype. Acta Biomaterialia. 2021.

CP Monckton, A Brougham-Cook, KB Kaylan, GH Underhill, SR Khetani. Elucidating Extracellular Matrix and Stiffness Control of Primary Human Hepatocyte Phenotype via Cell Microarrays. Advanced Materials Interfaces. 2021.

IC Berg, E Mohagheghian, K Habing, N Wang, GH Underhill. Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D. Advanced Healthcare Materials. Advanced Healthcare Materials. 2021.


SD Gentile, AP Kourouklis, H Ryoo, GH Underhill. Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids. Frontiers in Bioengineering and Biotechnology. 2020.


GH Underhill, SR Khetani. Emerging trends in modeling human liver disease in vitro. APL Bioengineering. 2019.

IC Berg, GH Underhill. High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays. Bio-protocol. 2019. DOI:


KB Kaylan, IC Berg, MJ Biehl, A Brougham-Cook, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling. Elife. 2018.

GH Underhill, SR Khetani. Advances in Engineered Human Liver Platforms for Drug Metabolism Studies. Drug Metabolism and Disposition. DOI:

MJ Biehl, KB Kaylan, RJ Thompson, RV Gonzalez, KE Weis, GH Underhill, LT Raetzman. Cellular fate decisions in the developing female anteroventral periventricular nucleus are regulated by canonical Notch signaling. Developmental Biology. 2018.

SD Gentile, ME Griebel, EW Anderson, GH Underhill. Click Chemistry-Based DNA Labeling of Cells for Barcoding Applications. Bioconjugate Chemistry. 2018.

M Gapinske, N Tague, J Winter, GH Underhill, P Perez-Pinera. Targeted Gene Knock Out Using Nuclease-Assisted Vector Integration: Hemi- and Homozygous Deletion of JAG1. Synthetic Biology. 2018.

GH Underhill, SR Khetani. Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies. Cellular and Molecular Gastroenterology and Hepatology. 2018.


RCA Eguiluz, KB Kaylan, GH Underhill, DE Leckband. Substrate stiffness and VE-cadherin mechano-transduction coordinate to regulate endothelial monolayer integrity. Biomaterials. 2017.

KB Kaylan, AP Kourouklis, GH Underhill. A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces. JoVE. 2017. DOI: 10.3791/55362


KB Kaylan, SD Gentile, LE Milling, KN Bhinge, F Kosari, GH Underhill. Mapping lung tumor cell drug responses as a function of matrix context and genotype using cell microarrays. Integrative Biology. 2016.

AP Kourouklis, KB Kaylan, GH Underhill. Substrate stiffness and matrix composition coordinately control the differentiation of liver progenitor cells. Biomaterials. 2016.

DFB Malta, NE Reticker-Flynn, CL Da Silva, JMS Cabral, HE Fleming, KS Zaret, SN Bhatia, GH Underhill. Extracellular matrix microarrays to study inductive signaling for endoderm specification. Acta Biomaterialia. 2016.

KB Kaylan, V Ermilova, RC Yada, GH Underhill. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFβ and extracellular matrix. Sci Rep 6, 23490. 2016.